Primary Culture of Identified Neurones from a Cnidarian
نویسندگان
چکیده
منابع مشابه
Growth and synapse formation by identified leech neurones in culture: a review.
Within hours after identified neurones have been isolated from the CNS of the leech, they begin to sprout and to form synapses. Electrical recordings made by loose-patch clamp show that the tip of the isolated neurone has distinct properties with a high density of sodium channels. Neurites grow out from this tip after about 30 min and continue to grow for the next few days. The extent of growth...
متن کاملMembrane properties and selective connexions of identified leech neurones in culture.
1. Individual, identified neurones, dissected from the central nervous system of the leech and maintained in culture for several weeks, sprouted processes and formed synaptic connexions.2. The action potentials of isolated touch (T), pressure (P), nociceptive (N) cells and Retzius cells resembled those of their counterparts in situ, enabling them to be recognized unambiguously. Their input resi...
متن کاملNeurite outgrowth and synapse formation by identified leech neurones in culture.
After injury, neurones in the central nervous system (CNS) of the leech regenerate with a high degree of specificity. The aim of our experiments has been to study the sequential steps involved in neurite growth and synapse formation using isolated identified neurones in culture. An important requirement for sprouting of leech neurones is the substrate. Neurites grow only slowly and sparsely on ...
متن کاملNa+, K+ and Ca2+ currents in identified leech neurones in culture.
1. Na+, K+ and Ca2+ currents have been measured by voltage-clamp in Retzius (R), anterior pagoda (AP) and sensory (pressure, touch and nociceptive) cells dissected from the central nervous system (CNS) of the leech. These cells maintain their distinctive membrane properties and action potential configurations in culture. Currents carried by the individual ions were analysed by the use of channe...
متن کاملPrimary culture of crustacean stomatogastric ganglion neurones in a defined medium.
The crustacean stomatogastric ganglion, consisting of about 30 identifiable neurones, produces several distinct patterned outputs governing rhythmic movements of the stomach. This system has become a model for addressing cellular properties and synaptic interactions underlying pattern generation in neural networks (see Selverston and Moulins, 1987). Studies of the system have been limited by th...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 1989
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.142.1.97